Deleted
Sample Discontinued
Experience: None
Not Used
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Reporter

Part:BBa_K3176000:Design

Designed by: Tara Fournier   Group: iGEM19_Pasteur_Paris   (2019-10-15)


mRFP for S.aureus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 597
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design of BBa_K3176000, we had to change the promoter of BBa_J04450 from LacI to pMK4Pprot.


Source

This part was adapted from the biobrick BBa_J04450

References

Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent reporters for Staphylococcus aureus. Journal of microbiological methods, 77(3), 251–260. doi:10.1016/j.mimet.2009.02.011